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• Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis (H 0 ): There’s no effect in the population .
• Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question
• They both make claims about the population
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
• Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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• Knowledge Base

Methodology

• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Alternative hypothesis

by Marco Taboga , PhD

In a statistical test, observed data is used to decide whether or not to reject a restriction on the data-generating probability distribution.

The assumption that the restriction is true is called null hypothesis , while the statement that the restriction is not true is called alternative hypothesis.

A correct specification of the alternative hypothesis is essential to decide between one-tailed and two-tailed tests.

Table of contents

Mathematical setting

Choice between one-tailed and two-tailed tests, the critical region, the interpretation of the rejection, the interpretation must be coherent with the alternative hypothesis.

• Power function

Accepting the alternative

More details, keep reading the glossary.

In order to fully understand the concept of alternative hypothesis, we need to remember the essential elements of a statistical inference problem:

we observe a sample drawn from an unknown probability distribution;

in principle, any valid probability distribution could have generated the sample;

however, we usually place some a priori restrictions on the set of possible data-generating distributions;

A couple of simple examples follow.

When we conduct a statistical test, we formulate a null hypothesis as a restriction on the statistical model.

The alternative hypothesis is

The alternative hypothesis is used to decide whether a test should be one-tailed or two-tailed.

The null hypothesis is rejected if the test statistic falls within a critical region that has been chosen by the statistician.

The critical region is a set of values that may comprise:

only the left tail of the distribution or only the right tail (one-tailed test);

both the left and the right tail (two-tailed test).

The choice of the critical region depends on the alternative hypothesis. Let us see why.

The interpretation is different depending on the tail of the distribution in which the test statistic falls.

The choice between a one-tailed or a two-tailed test needs to be done in such a way that the interpretation of a rejection is always coherent with the alternative hypothesis.

When we deal with the power function of a test, the term "alternative hypothesis" has a special meaning.

We conclude with a caveat about the interpretation of the outcome of a test of hypothesis.

The interpretation of a rejection of the null is controversial.

According to some statisticians, rejecting the null is equivalent to accepting the alternative.

However, others deem that rejecting the null does not necessarily imply accepting the alternative. In fact, it is possible to think of situations in which both hypotheses can be rejected. Let us see why.

According to the conceptual framework illustrated by the images above, there are three possibilities:

the null is true;

the alternative is true;

neither the null nor the alternative is true because the true data-generating distribution has been excluded from the statistical model (we say that the model is mis-specified).

If we are in case 3, accepting the alternative after a rejection of the null is an incorrect decision. Moreover, a second test in which the alternative becomes the new null may lead us to another rejection.

You can find more details about the alternative hypothesis in the lecture on Hypothesis testing .

Previous entry: Almost sure

Next entry: Binomial coefficient

How to cite

Please cite as:

Taboga, Marco (2021). "Alternative hypothesis", Lectures on probability theory and mathematical statistics. Kindle Direct Publishing. Online appendix. https://www.statlect.com/glossary/alternative-hypothesis.

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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10.1 - setting the hypotheses: examples.

A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or differences between means or proportions or correlations or odds ratios or any other numerical summary of the population. The alternative hypothesis is typically the research hypothesis of interest. Here are some examples.

Example 10.2: Hypotheses with One Sample of One Categorical Variable Section  

About 10% of the human population is left-handed. Suppose a researcher at Penn State speculates that students in the College of Arts and Architecture are more likely to be left-handed than people found in the general population. We only have one sample since we will be comparing a population proportion based on a sample value to a known population value.

• Research Question : Are artists more likely to be left-handed than people found in the general population?
• Response Variable : Classification of the student as either right-handed or left-handed

State Null and Alternative Hypotheses

• Null Hypothesis : Students in the College of Arts and Architecture are no more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Art and Architecture = 10% or p = .10).
• Alternative Hypothesis : Students in the College of Arts and Architecture are more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Arts and Architecture > 10% or p > .10). This is a one-sided alternative hypothesis.

Example 10.3: Hypotheses with One Sample of One Measurement Variable Section  

A generic brand of the anti-histamine Diphenhydramine markets a capsule with a 50 milligram dose. The manufacturer is worried that the machine that fills the capsules has come out of calibration and is no longer creating capsules with the appropriate dosage.

• Research Question : Does the data suggest that the population mean dosage of this brand is different than 50 mg?
• Response Variable : dosage of the active ingredient found by a chemical assay.
• Null Hypothesis : On the average, the dosage sold under this brand is 50 mg (population mean dosage = 50 mg).
• Alternative Hypothesis : On the average, the dosage sold under this brand is not 50 mg (population mean dosage ≠ 50 mg). This is a two-sided alternative hypothesis.

Example 10.4: Hypotheses with Two Samples of One Categorical Variable Section  

Many people are starting to prefer vegetarian meals on a regular basis. Specifically, a researcher believes that females are more likely than males to eat vegetarian meals on a regular basis.

• Research Question : Does the data suggest that females are more likely than males to eat vegetarian meals on a regular basis?
• Response Variable : Classification of whether or not a person eats vegetarian meals on a regular basis
• Explanatory (Grouping) Variable: Sex
• Null Hypothesis : There is no sex effect regarding those who eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis = population percent of males who eat vegetarian meals on a regular basis or p females = p males ).
• Alternative Hypothesis : Females are more likely than males to eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis > population percent of males who eat vegetarian meals on a regular basis or p females > p males ). This is a one-sided alternative hypothesis.

Example 10.5: Hypotheses with Two Samples of One Measurement Variable Section  

Obesity is a major health problem today. Research is starting to show that people may be able to lose more weight on a low carbohydrate diet than on a low fat diet.

• Research Question : Does the data suggest that, on the average, people are able to lose more weight on a low carbohydrate diet than on a low fat diet?
• Response Variable : Weight loss (pounds)
• Explanatory (Grouping) Variable : Type of diet
• Null Hypothesis : There is no difference in the mean amount of weight loss when comparing a low carbohydrate diet with a low fat diet (population mean weight loss on a low carbohydrate diet = population mean weight loss on a low fat diet).
• Alternative Hypothesis : The mean weight loss should be greater for those on a low carbohydrate diet when compared with those on a low fat diet (population mean weight loss on a low carbohydrate diet > population mean weight loss on a low fat diet). This is a one-sided alternative hypothesis.

Example 10.6: Hypotheses about the relationship between Two Categorical Variables Section  

• Research Question : Do the odds of having a stroke increase if you inhale second hand smoke ? A case-control study of non-smoking stroke patients and controls of the same age and occupation are asked if someone in their household smokes.
• Variables : There are two different categorical variables (Stroke patient vs control and whether the subject lives in the same household as a smoker). Living with a smoker (or not) is the natural explanatory variable and having a stroke (or not) is the natural response variable in this situation.
• Null Hypothesis : There is no relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is = 1).
• Alternative Hypothesis : There is a relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is > 1). This is a one-tailed alternative.

This research question might also be addressed like example 11.4 by making the hypotheses about comparing the proportion of stroke patients that live with smokers to the proportion of controls that live with smokers.

Example 10.7: Hypotheses about the relationship between Two Measurement Variables Section  

• Research Question : A financial analyst believes there might be a positive association between the change in a stock's price and the amount of the stock purchased by non-management employees the previous day (stock trading by management being under "insider-trading" regulatory restrictions).
• Variables : Daily price change information (the response variable) and previous day stock purchases by non-management employees (explanatory variable). These are two different measurement variables.
• Null Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) = 0.
• Alternative Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) > 0. This is a one-sided alternative hypothesis.

Example 10.8: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples Section  

• Research Question : Is there a linear relationship between the amount of the bill (\$) at a restaurant and the tip (\$) that was left. Is the strength of this association different for family restaurants than for fine dining restaurants?
• Variables : There are two different measurement variables. The size of the tip would depend on the size of the bill so the amount of the bill would be the explanatory variable and the size of the tip would be the response variable.
• Null Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the same at family restaurants as it is at fine dining restaurants.
• Alternative Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the difference at family restaurants then it is at fine dining restaurants. This is a two-sided alternative hypothesis.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

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Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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Once you have developed a clear and focused research question or set of research questions, you’ll be ready to conduct further research, a literature review, on the topic to help you make an educated guess about the answer to your question(s). This educated guess is called a hypothesis.

In research, there are two types of hypotheses: null and alternative. They work as a complementary pair, each stating that the other is wrong.

• Null Hypothesis (H 0 ) – This can be thought of as the implied hypothesis. “Null” meaning “nothing.”  This hypothesis states that there is no difference between groups or no relationship between variables. The null hypothesis is a presumption of status quo or no change.
• Alternative Hypothesis (H a ) – This is also known as the claim. This hypothesis should state what you expect the data to show, based on your research on the topic. This is your answer to your research question.

Null Hypothesis:   H 0 : There is no difference in the salary of factory workers based on gender. Alternative Hypothesis :  H a : Male factory workers have a higher salary than female factory workers.

Null Hypothesis :  H 0 : There is no relationship between height and shoe size. Alternative Hypothesis :  H a : There is a positive relationship between height and shoe size.

Null Hypothesis :  H 0 : Experience on the job has no impact on the quality of a brick mason’s work. Alternative Hypothesis :  H a : The quality of a brick mason’s work is influenced by on-the-job experience.

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Neag School of Education

Educational Research Basics by Del Siegle

Null and alternative hypotheses.

Converting research questions to hypothesis is a simple task. Take the questions and make it a positive statement that says a relationship exists (correlation studies) or a difference exists between the groups (experiment study) and you have the alternative hypothesis. Write the statement such that a relationship does not exist or a difference does not exist and you have the null hypothesis. You can reverse the process if you have a hypothesis and wish to write a research question.

When you are comparing two groups, the groups are the independent variable. When you are testing whether something affects something else, the cause is the independent variable. The independent variable is the one you manipulate.

Teachers given higher pay will have more positive attitudes toward children than teachers given lower pay. The first step is to ask yourself “Are there two or more groups being compared?” The answer is “Yes.” What are the groups? Teachers who are given higher pay and teachers who are given lower pay. The independent variable is teacher pay. The dependent variable (the outcome) is attitude towards school.

You could also approach is another way. “Is something causing something else?” The answer is “Yes.”  What is causing what? Teacher pay is causing attitude towards school. Therefore, teacher pay is the independent variable (cause) and attitude towards school is the dependent variable (outcome).

By tradition, we try to disprove (reject) the null hypothesis. We can never prove a null hypothesis, because it is impossible to prove something does not exist. We can disprove something does not exist by finding an example of it. Therefore, in research we try to disprove the null hypothesis. When we do find that a relationship (or difference) exists then we reject the null and accept the alternative. If we do not find that a relationship (or difference) exists, we fail to reject the null hypothesis (and go with it). We never say we accept the null hypothesis because it is never possible to prove something does not exist. That is why we say that we failed to reject the null hypothesis, rather than we accepted it.

Del Siegle, Ph.D. Neag School of Education – University of Connecticut [email protected] www.delsiegle.com

• Alternative vs Null Hypothesis: Pros, Cons, Uses & Examples

All research starts with a problem that needs to be solved. From this problem, hypotheses are developed to provide the researcher with a clear statement of the problem.

To understand alternative hypotheses also known as alternate hypotheses, you must first understand what the hypothesis is .

When you hear the word hypothesis it means the accurate explanations in relation to a set of facts that can be analyzed when studied, using some specific method of research.

There are primarily two types of hypothesis which are null hypothesis and alternative hypothesis.

When you think about the word “null” what should come to mind is something that can not change, what you expect is what you get, unlike alternate hypotheses which can change.

Now, the research problems or questions which could be in the form of null hypothesis or alternative hypothesis are expressed as the relationship that exists between two or more variables. The process for this states that the questions should be what expresses the relationship between two variables that can be measured.

Both null hypotheses and alternative hypotheses are used by statisticians and researchers to conduct research in various industries or fields such as mathematics, psychology, science, medicine, and technology.

We are going to discuss alternative hypotheses and null hypotheses in this post and how they work in research.

What is an Alternative Hypothesis?

Alternative hypothesis simply put is another viable option to the null hypothesis. It means looking for a substantial change or option that can allow you to reject the null hypothesis.

It is an opposing theory to a null hypothesis.

If you develop a null hypothesis, you make an informed guess on whether a thing is true or whether there is a relationship between that thing and another variable. An alternate hypothesis will always take an opposite stand against a null hypothesis. So if according to a null hypothesis something is correct to an alternate hypothesis that same thing will be incorrect.

For example, let’s assume that you develop a null hypothesis that states “I”m going to be $500 richer” the alternate hypothesis will be “I’m going to get $500 or be richer”

When you are trying to disprove a null hypothesis, that is when you test an alternate hypothesis. If there is enough data to back up the alternative hypothesis then you can dispose of the null hypothesis. 

Get Answers: What is Empirical Research Study? [Examples & Method]

What is a Null Hypothesis?

The null hypothesis is best explained as the statement showing that no relationship exists between two variables that are being considered or that two groups are not related. As we have earlier established, a hypothesis is an assumed statement that has not been proven with sufficient data that could serve as a piece of evidence. 

The null hypothesis is now the statement that a researcher or an investigator wants to disprove. The null hypothesis is capable of being tested, being verifiable, and also capable of being rejected.

For example, if you want to conduct a study that will compare the relationship between project A and project B if the study is based on the assumption that both projects are of equal standard, the assumption is referred to as the null hypothesis.

This is because the null hypothesis should be specific at all times.

Learn: Hypothesis Testing in Research: Definition, Procedure, Uses, Limitations + Examples

Advantages of the Alternative Hypothesis 

• Alternative hypothesis gives a researcher specific clarifications on the research questions or problems .
• It provides a study with the direction that can be used to collect data and obtain results of interest by the researcher.
• An alternative hypothesis is always selected before commencing the studies which gives the researcher the opportunity to prove that the restatement is backed up by evidence and not just from the researcher’s ideas or values.
• Another good thing about alternative hypotheses is that it provides the opportunity to discover new theories that a researcher can use to disprove an existing theory that may not have been backed up by evidence .
• An alternate hypothesis is also useful to prove that there is a relationship between two selected variables and the outcomes of the conducted study are relevant.

Principles of the Alternative Hypothesis

• Alternative hypotheses will be accepted if the amount of data that is gone is insignificant within the significance level. This means that the null hypothesis will be rejected.
• Another principle of the alternative hypothesis is that the data gathered from random samples go through a statistical tool that analyzes the effect of the amount of data leaving the null hypothesis.

For the curious: Sampling Bias: Definition, Types + [Examples]

Purpose of the Null Hypothesis 

Here are the purposes of the null hypothesis in an experiment or study:

• The primary purpose of a null hypothesis is to disprove an assumption.
• Null hypotheses can help to further progress a theory in some scientific cases.
• You can also use a null hypothesis to ascertain how consistent the outcomes of multiple studies are.

Principle of the Null Hypothesis 

Now, these are the principles of the null hypothesis:

1. The primary principle of the null hypothesis is to prove that the assumed statement is true. This is done by collecting data and analyzing in the study , what chance the collected data has in the random sample.

2. If the collected data does not meet the expectation of the null hypothesis, it is determined that the data lacks sufficient evidence to back up the null hypothesis therefore the null hypothesis statement is rejected.

Just as in the case of the alternative hypothesis the collected data in a null hypothesis is analyzed using some statistical tools that are made to measure the extent to which data left the null hypothesis.

The process will determine whether the data that left the null hypothesis is larger than a set value. If the data collected from the random sample is enough to serve as evidence to prove the null hypothesis then the null hypothesis will be accepted as true. And also defined that it has no relationship with other variables .

Learn About: Research Reports: Definition, Types + [Writing Guide]

Types of Alternative Hypothesis (Advantages of Each and When to Use)

There are four types of alternative hypotheses, and we will briefly discuss them below.

• One-tailed directional: For one of the sampling distributions one tail, this type of alternative hypothesis focuses on the rejected part only.
• Two-tailed directional: In an alternative hypothesis, a two-tailed directional focus on the two parts or directions that were rejected in the sampling distribution.
• Point: Point is another alternate hypothesis. It occurs in hypothesis testing when the sample population in the alternate hypothesis has been completely defined in a distribution. If there are no known parameters, the hypothesis will serve no interest. They are, however, important to the foundation of the statistical inferences.
• Non-directional: In an alternate hypothesis, a non-directional does not focus on the two directions of rejection. The only focus of the nondirectional alternative hypothesis is to prove that the null hypothesis is incorrect.

Read: Type I vs Type II Errors: Definition, Examples & Prevention

Difference between Null Hypothesis and Alternative Hypothesis 

We are going to look at the differences between the alternate hypothesis and the null hypothesis based on these six factors which are:

• Mathematical expression
• Observation
• Acceptance criteria
• The difference in Mathematical expression

Null hypothesis is followed by an ‘equals to’ (=) sign. While the Alternative hypothesis is followed by these three signs; 

• The difference in how they are observed

In the null hypothesis, it is believed that the results that are observed are as a result of chance. While In the alternative hypothesis, it is believed that the observed results are the outcome of some real causes.

• Differences in results

The result of the null hypothesis always shows that there have been no changes in statements or opinions. While the result of the alternative hypothesis shows that there have been significant changes in statements and opinions.

• Differences in Acceptance criteria

If the p-value in a null hypothesis is greater than the significance level, then the null hypothesis is accepted.

If the p-value in an alternate hypothesis is smaller than the significance level, then the alternative hypothesis is accepted.

• Differences in importance

The null hypothesis accepts true existing theories and also if there has been consistency in multiple experiments of similar hypotheses.

The alternative hypothesis establishes whether a relationship exists between two variables, and the result will then lead to new improved theories.

Read: T-testing: Definition, Formula & Interpretations

Examples of an Alternative Hypothesis and Null Hypothesis

Here are some examples of the alternative hypothesis:.

A researcher assumes that a bridge’s bearing capacity is over 10 tons, the researcher will then develop an hypothesis to support this study. The hypothesis will be:

For the null hypothesis H0: µ= 10 tons

For the alternate hypothesis Ha: µ>10 tons

In another study being conducted, the researcher wants to find out whether there is a noticeable difference or change in a patient’s heart arrest medicine and the patient’s heart condition.

For the alternate hypothesis: The hypothesis is that there might indeed be a relationship between the new medicine and the frequency or chances of heart arrest in a patient.

Here are the examples of the null hypothesis

The hypothesis from example 2 in the alternate hypothesis implies that the use of one specific medicine can reduce the frequency and chances of heart arrest.

For the null hypothesis: The hypothesis will be that the use of that particular medicine cannot reduce the chance and frequency of heart arrest in a patient.

An alternate hypothesis states that the random exam scores are collected from both men and women. But are the scores of the two groups (men and women) the same or are they different?

For the null hypothesis: The hypothesis will state that the calculated mean of the men’s exam score is equal to the exam score of the women.

This is represented as

H0= The null hypothesis

µ1= The calculated mean score of men

µ2= The calculated mean score of women

Read: What is Empirical Research Study? [Examples & Method]

• Can you reject an alternative hypothesis?

It is quite inappropriate to say or report that an alternate hypothesis was rejected. It is much better to use the phrase “the alternate hypothesis was rather not supported”.

The reason behind this use of words is that only the null hypothesis is designed to be rejected in a study. The alternative hypothesis is designed to prove the null hypothesis incorrect, to introduce new facts that can disprove the null hypothesis but it is not designed to be rejected.

It can either be accepted or not supported.

• How do you identify alternative hypotheses?

A researcher can use this formula to identify the alternate hypothesis in a study or experiment.

H0 and Ha are in contrast.

Therefore, if Ho has:

Equal to (=)

Greater than or equal to (≥)

Less than or equal to (≤)

And then Ha has:

Not equal (≠) 

Greater than (>) or less than (

Less than ( )

If in a study, α ≤ p-value, then the researcher should not reject H0.

If in a study, α > p-value, then the researcher should reject H0.

α is preconceived. The value of α is determined even before the hypothesis test is conducted. While the p-value is derived from the calculation in the data.

• Which is better in formulating hypotheses of your study alternative or null?

The study a researcher wants to conduct will determine what hypothesis should be developed. However, the researcher should keep in mind what the purpose of the null and alternative two hypotheses are while developing the study hypothesis. So while the null hypothesis will accept existing theories that it found to be true or correct, and measure the consistency of multiple experiments, alternative hypotheses will find the relationship that exists (if any) between two phenomena and may lead to the development of a new and improved theory.

In this article, it has been clearly defined the relationship that exists between the null hypothesis and the alternative hypothesis. While the null hypothesis is always an assumption that needs to be proven with evidence for it to be accepted, the alternative hypothesis puts in all the effort to make sure the null hypothesis is disproved. 

Researchers should note that for every null hypothesis, one or more alternate hypotheses can be developed.

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How to Write a Research Hypothesis: Good & Bad Examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Bad Hypothesis Examples

Tips for Writing a Research Hypothesis

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

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Educational resources and simple solutions for your research journey

What is a Research Hypothesis: How to Write it, Types, and Examples

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

• Clearly formulated and free of language errors and ambiguity  
• Concise and not unnecessarily verbose  
• Has clearly defined variables  
• Testable and stated in a way that allows for it to be disproven  
• Can be tested using a research design that is feasible, ethical, and practical   
• Specific and relevant to the research problem  
• Rooted in a thorough literature search  
• Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

• Identify a research problem or question: Start by identifying a specific research problem.   
• Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
• Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
• State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
• Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
• Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

• Testable: A research hypothesis should be able to be tested via experimentation or observation.  
• Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
• Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
• Falsifiable: A research hypothesis should be able to be disproven through testing.  
• Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
• Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
• Relevant: A research hypothesis should be relevant to the research question and objectives.  
• Feasible: A research hypothesis should be feasible to test within the scope of the study.  
• Reflects the population: A research hypothesis should consider the population or sample being studied.  
• Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

• Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
• The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
• You should be able to collect the necessary data within the constraints of your study.  
• It should be possible for other researchers to replicate your study, using the same methods and variables.   
• Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
• The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

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Alternative Hypothesis

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Diving deep into the realm of scientific research, the alternative hypothesis plays a pivotal role in steering investigations. It stands contrary to the null hypothesis , providing a different perspective or direction. This essential component often sets the foundation for groundbreaking discoveries. If you’re keen on understanding this concept further, our collection of alternative hypothesis statement examples, combined with a thorough writing guide and insightful tips, will serve as your comprehensive roadmap.

What is an Alternative hypothesis?

An alternative hypothesis is a statement used in statistical testing that indicates the presence of an effect, relationship, or difference. It stands in direct contrast to the null hypothesis, which posits that there is no effect or relationship. The alternative causual hypothesis provides a specific direction to the research and can be directional (e.g., one value is greater than another) or non-directional (e.g., two values are not equal).

What is an example of an Alternative hypothesis statement?

If a researcher is studying the effect of a new teaching method on student performance, the null hypothesis might be: “The new teaching method has no effect on student performance.” An example of an alternative hypothesis could be:

Directional: “Students exposed to the new teaching method will perform better than those who were not.” Non-directional: “Student performance will be different for those exposed to the new teaching method compared to those who were not.”

100 Alternative Hypothesis Statement Examples

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The alternative hypothesis symbolizes a statement of what a statistical hypothesis test is set to establish. Often contrasted with a null hypothesis, it indicates the expected direction of the tested relation. Dive into these varied thesis statement examples showcasing the core essence of alternative hypotheses.

• Smoking and Cancer : Smoking is positively related to lung cancer incidence.
• Diet and Weight Loss : The Atkins diet results in more weight loss than a conventional diet.
• Medication Efficiency : Drug A is more effective than Drug B in treating migraines.
• Exercise Duration : Engaging in physical activity for more than 30 minutes daily reduces depression symptoms.
• Class Size and Learning : Smaller class sizes lead to higher student test scores.
• Sugar Intake : Consuming more than 50 grams of sugar daily increases the risk of diabetes.
• Vitamin C and Cold : Vitamin C intake reduces the duration of the common cold.
• Sleep Duration : Sleeping less than 6 hours results in decreased cognitive function.
• Training Methods : Method X training increases employee productivity more than Method Y.
• Pollution Levels : Higher levels of industrial activity correlate with increased air pollution.
• Stress and Disease : Chronic stress has a positive relationship with heart diseases.
• Alcohol and Reaction Time : Alcohol consumption slows down reaction time.
• Meditation and Blood Pressure : Regular meditation lowers blood pressure.
• Organic Food : Consuming organic food leads to better gut health.
• Advertising : Increased advertising results in higher sales figures.
• Salary and Job Satisfaction : A higher salary correlates with job satisfaction.
• Age and Memory : As age increases, short-term memory retention decreases.
• Temperature and Aggression : Higher temperatures are associated with increased aggressive behavior.
• Social Media : Spending more than 2 hours on social media daily increases feelings of loneliness.
• Music and Concentration : Listening to classical music improves concentration during studies. …
• Recycling Habits : Communities with mandatory recycling policies have higher recycling rates.
• Urban Areas : Living in urban areas increases the likelihood of asthma.
• Pets and Loneliness : Owning a pet decreases feelings of loneliness.
• Reading Habits : Reading more than 3 books a month correlates with increased empathy.
• Green Spaces : Having access to green spaces reduces stress levels.
• Vaccination : Vaccination reduces the incidence of specific diseases.
• Chocolate and Mood : Consuming chocolate elevates mood.
• Remote Work : Working remotely improves overall work satisfaction.
• Financial Literacy : Financial literacy education reduces personal debt.
• Mindfulness and Anxiety : Practicing mindfulness decreases symptoms of anxiety. …
• Dietary Fiber : Higher dietary fiber intake is associated with lower risks of bowel cancer.
• Travel and Creativity : People who travel frequently are more creative.
• Education Level and Income : Individuals with higher education levels earn more income.
• Technology Adoption : People who receive technology training adapt to new devices faster.
• Parental Involvement and Academic Performance : Increased parental involvement enhances students’ academic performance.
• Exercise Frequency and Heart Health : Exercising at least five times a week improves heart health.
• Gender and Leadership Roles : Men are more likely to hold leadership positions in corporate settings.
• Social Support and Mental Health : Strong social support networks reduce the risk of depression.
• Quality of Sleep and Productivity : Better sleep quality leads to higher productivity levels.
• High-Fat Diet and Cholesterol Levels : A high-fat diet increases cholesterol levels.
• Caffeine Intake and Alertness : Higher caffeine intake enhances alertness and cognitive function.
• Online Shopping Habits : People who frequently shop online spend more money than in-store shoppers. …
• Education and Political Views : Higher education levels are associated with more liberal political views.
• Gender and Risk-Taking Behavior : Men are more likely to engage in risky behaviors.
• Temperature and Ice Cream Sales : Higher temperatures increase ice cream sales.
• Artificial Sweeteners and Weight Loss : Consuming products with artificial sweeteners aids in weight loss.
• Exercise and Stress Reduction : Regular exercise reduces stress levels.
• Music Genres and Mood : Listening to upbeat music improves mood.
• Online Learning and Engagement : Online learners are more engaged in virtual classroom discussions.
• Personality Traits and Job Performance : Extroverted individuals perform better in sales roles.
• Environmental Awareness and Recycling : Higher environmental awareness leads to more recycling practices.
• Social Media Usage and Self-Esteem : Excessive social media usage correlates with lower self-esteem. …
• Sleep Deprivation and Reaction Time : Sleep-deprived individuals have slower reaction times.
• Breakfast Consumption and Metabolism : Eating breakfast kickstarts metabolism for the day.
• Leadership Style and Employee Satisfaction : Transformational leadership style increases employee job satisfaction.
• Bilingualism and Cognitive Abilities : Bilingual individuals possess enhanced cognitive abilities.
• Video Game Playing and Aggression : Playing violent video games increases aggressive behavior.
• Hydration and Cognitive Function : Staying hydrated improves cognitive function.
• Parental Support and Academic Achievement : Supportive parenting leads to higher academic achievement.
• Workplace Flexibility and Work-Life Balance : Jobs with flexible schedules enhance work-life balance.
• Digital Learning and Knowledge Retention : Digital learning methods improve long-term knowledge retention.
• Art Exposure and Creativity : Exposure to various forms of art fosters creative thinking.
• Solar Energy Adoption and Utility Bills : Homes with solar energy systems experience lower utility bills.
• Parental Involvement and Student Behavior : Increased parental involvement reduces student behavioral issues.
• Team Diversity and Creativity : Diverse teams generate more creative solutions.
• Social Media Marketing and Brand Awareness : Social media marketing boosts brand awareness more than traditional methods.
• Morning Routine and Productivity : Following a structured morning routine enhances overall productivity.
• Music Training and Cognitive Development : Music training improves cognitive abilities in children.
• Employee Training and Job Satisfaction : Comprehensive employee training programs lead to higher job satisfaction.
• Eating Before Bed and Sleep Quality : Consuming heavy meals before bed negatively affects sleep quality.
• Financial Incentives and Employee Performance : Offering financial incentives increases employee performance.
• Parental Attachment and Emotional Well-being : Strong parental attachment fosters better emotional well-being in children.
• Social Interaction and Mental Well-being : Frequent social interaction correlates with improved mental health.
• Education and Crime Rates : Higher education levels result in lower crime rates within communities.
• Diet and Acne : A diet high in dairy products exacerbates acne.
• Leadership Style and Employee Motivation : Autocratic leadership style hampers employee motivation.
• Urban Green Spaces and Stress Reduction : Access to urban green spaces lowers stress levels.
• Sleep Duration and Athletic Performance : Adequate sleep duration enhances athletic performance.
• Financial Literacy and Investment Success : Individuals with high financial literacy make more successful investments.
• Team Collaboration and Project Success : Effective team collaboration leads to more successful project outcomes.
• Media Exposure and Body Image : Increased media exposure contributes to negative body image perceptions.
• Gender Representation and Film Success : Movies with more balanced gender representation achieve higher box office success. …
• Meditation and Anxiety Reduction : Regular meditation practice reduces symptoms of anxiety.
• Cognitive Training and Memory Enhancement : Cognitive training programs improve memory retention.
• Positive Affirmations and Self-Confidence : Repeating positive affirmations enhances self-confidence.
• Physical Fitness and Longevity : Being physically fit is linked to increased lifespan.
• Parental Guidance and Online Safety : Strong parental guidance promotes responsible online behavior in children.
• Artificial Intelligence and Job Displacement : Increased AI integration leads to more job displacement.
• Public Transportation Usage and Air Quality : Increased public transportation usage improves air quality in cities.
• Social Support and Addiction Recovery : Strong social support networks aid in addiction recovery.
• Gender Diversity and Company Performance : Companies with diverse gender representation outperform others.
• Mindfulness Meditation and Pain Management : Mindfulness meditation reduces perception of pain.
• Music Therapy and Autism : Music therapy improves social interaction skills in children with autism.
• Social Media Usage and Academic Performance : Excessive social media usage negatively impacts academic performance.
• Employee Engagement and Organizational Success : Higher employee engagement leads to greater organizational success.
• Healthy Eating and Longevity : A diet rich in fruits and vegetables contributes to a longer lifespan.
• Gender Stereotypes and Career Choice : Gender stereotypes influence career choices among young adults.
• Environmental Conservation Efforts and Biodiversity : Increased conservation efforts positively affect biodiversity.
• Volunteerism and Personal Well-being : Engaging in volunteer activities enhances personal well-being.
• Artificial Intelligence and Customer Service : AI-driven customer service improves user satisfaction.

Alternative Hypothesis Statement Examples in Research

In alternative research hypothesis propel investigations beyond the null. Examples span diverse fields, revealing the direction researchers expect their findings to take.

• Effect of Music on Concentration : Listening to classical music enhances concentration during study.
• Green Tea and Weight Loss : Green tea consumption leads to more significant weight loss than water intake.
• Parental Involvement and Academic Achievement : Active parental involvement boosts student academic achievement.
• Social Media Usage and Self-Esteem : Frequent social media use correlates with lower self-esteem.

Alternative Hypothesis Statement Examples in Business Research

Business research thrives on alternative hypotheses. Dive into these business-oriented examples that challenge null assumptions.

• Marketing Campaign Impact : Marketing campaign A generates higher conversion rates than campaign B.
• Employee Training and Productivity : Comprehensive employee training enhances workplace productivity.
• Work-Life Balance and Employee Satisfaction : Improved work-life balance increases employee job satisfaction.
• Customer Service Channel Effectiveness : Online chat support results in higher customer satisfaction compared to phone support.
• Branding Influence on Purchase Intent : Strong brand presence leads to increased purchase intent.

Directional Alternative Hypothesis Statement Examples

Directional hypothesis add clarity to research expectations. Explore these examples that predict specific outcomes.

• Exercise Frequency and Heart Health : Engaging in physical activity five times a week improves heart health.

Alternative Hypothesis Statement Examples in Psychology

Psychological studies benefit from well-crafted alternative hypotheses. These psychology hypothesis examples delve into the realm of human behavior and cognition.

• Mindfulness Meditation and Anxiety Reduction : Regular mindfulness practice reduces symptoms of anxiety.

Alternative Null Hypothesis Statement Examples

Explore alternative null hypothesis —statements asserting the absence of specific effects or differences.

• Coffee Consumption and Weight Gain : Increased coffee consumption does not lead to weight gain.
• Smartphone Usage and Sleep Quality : Using smartphones before bed does not impact sleep quality.
• Music Genre and Study Performance : Studying with rock music does not affect academic performance.
• Green Spaces and Stress Reduction : Access to green spaces does not decrease stress levels.
• Team Diversity and Project Success : Team diversity does not influence project success rates.

Alternative Hypothesis Statement Examples in Medical Research

Medical research relies on robust alternative hypotheses to drive scientific inquiry. These examples explore hypotheses in the realm of healthcare.

• Exercise and Diabetes Prevention : Regular exercise decreases the risk of developing type 2 diabetes.
• Medication A and Blood Pressure Reduction : Medication A leads to greater reduction in blood pressure compared to medication B.
• Nutritional Intake and Heart Disease : Higher intake of fruits and vegetables lowers the risk of heart disease.
• Stress Reduction Techniques and Anxiety Levels : Practicing stress reduction techniques decreases anxiety levels.
• Alternative Medicine and Pain Management : Alternative medicine therapies alleviate chronic pain more effectively than traditional treatments.

Alternative Hypothesis Statement Examples in Education Research

Education research thrives on alternative hypotheses to investigate innovative approaches. Explore examples that challenge conventional notions.

• Technology Integration and Student Engagement : Integrating technology enhances student engagement in the classroom.
• Project-Based Learning and Knowledge Retention : Project-based learning improves long-term knowledge retention.
• Teacher Professional Development and Student Performance : Effective teacher professional development positively impacts student academic performance.
• Inclusive Classroom Environment and Learning Outcomes : Inclusive classrooms lead to better learning outcomes for diverse students.
• Feedback Frequency and Writing Improvement : Frequent feedback results in greater improvement in student writing skills.

These examples showcase the pivotal role of alternative hypotheses across various disciplines, serving as the driving force behind scientific exploration and advancement.

What is the Alternative Hypothesis Formula?

The alternative hypothesis, denoted as “Ha” or “H1,” represents the assertion researchers aim to support through evidence. It stands in contrast to the null hypothesis (Ho), which suggests no effect or relationship. The formula for the alternative hypothesis varies based on the nature of the study:

• Directional Hypothesis : For studies with an expected direction, the formula takes the form of a prediction. For instance, “The new drug increases patient recovery rates.”
• Non-Directional Hypothesis : For exploratory studies, the formula reflects the possibility of any difference or effect. For example, “There is a difference in recovery rates between the two drugs.”

How do you start an Alternative Hypothesis?

Starting an alternative simple hypothesis involves framing a clear research statement that highlights the anticipated effect, relationship, or difference. To begin:

• Identify the Research Question: Determine the specific aspect you intend to explore or compare.
• Formulate a Hypothesis: Craft a statement that directly addresses the expected outcome.
• Include Variables: Introduce the relevant variables and their predicted connection.
• Be Clear and Specific: Ensure the alternative hypothesis is concise and unambiguous.

Is the Alternative Hypothesis a Claim or Statement?

The alternative hypothesis is both a claim and a statement. It claims that there is a measurable effect, relationship, or difference in the variables being studied. It is also a statement that researchers work to validate through evidence.

How do you write an Alternative Hypothesis Statement? – Step by Step Guide

Creating a robust alternative hypothesis statement involves structured steps:

• Identify Variables : Clearly define the independent and dependent variables in your study.
• State Expected Effect : Express the anticipated impact, relationship, or difference between variables.
• Be Precise : Use specific language to convey the exact nature of the expected outcome.
• Include Direction (if applicable) : If your hypothesis is directional, specify the expected direction.
• Avoid Ambiguity : Make sure your statement is clear and leaves no room for confusion.

Tips for Writing an Alternative Hypothesis Statement

• Be Specific : Clearly define the variables and the predicted relationship.
• Use Measurable Terms : Incorporate quantifiable terms to indicate the magnitude of the effect.
• Testability : Ensure the hypothesis can be tested empirically.
• Conciseness : Keep the statement concise and to the point.
• Alignment with Research Question : Ensure the hypothesis directly answers your research question.
• Avoid Value Judgments : Avoid value judgments or personal biases in the hypothesis.
• Review Literature : Consult existing literature to align your hypothesis with prior research.

Crafting a strong alternative hypothesis statement is essential for guiding your research and forming the basis for causual hypothesis testing. It directs the focus of your investigation and lays the foundation for drawing meaningful conclusions.

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• v.34(45); 2019 Nov 25

Scientific Hypotheses: Writing, Promoting, and Predicting Implications

Armen yuri gasparyan.

1 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, West Midlands, UK.

Lilit Ayvazyan

2 Department of Medical Chemistry, Yerevan State Medical University, Yerevan, Armenia.

Ulzhan Mukanova

3 Department of Surgical Disciplines, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

George D. Kitas

5 Arthritis Research UK Epidemiology Unit, University of Manchester, Manchester, UK.

Scientific hypotheses are essential for progress in rapidly developing academic disciplines. Proposing new ideas and hypotheses require thorough analyses of evidence-based data and predictions of the implications. One of the main concerns relates to the ethical implications of the generated hypotheses. The authors may need to outline potential benefits and limitations of their suggestions and target widely visible publication outlets to ignite discussion by experts and start testing the hypotheses. Not many publication outlets are currently welcoming hypotheses and unconventional ideas that may open gates to criticism and conservative remarks. A few scholarly journals guide the authors on how to structure hypotheses. Reflecting on general and specific issues around the subject matter is often recommended for drafting a well-structured hypothesis article. An analysis of influential hypotheses, presented in this article, particularly Strachan's hygiene hypothesis with global implications in the field of immunology and allergy, points to the need for properly interpreting and testing new suggestions. Envisaging the ethical implications of the hypotheses should be considered both by authors and journal editors during the writing and publishing process.

INTRODUCTION

We live in times of digitization that radically changes scientific research, reporting, and publishing strategies. Researchers all over the world are overwhelmed with processing large volumes of information and searching through numerous online platforms, all of which make the whole process of scholarly analysis and synthesis complex and sophisticated.

Current research activities are diversifying to combine scientific observations with analysis of facts recorded by scholars from various professional backgrounds. 1 Citation analyses and networking on social media are also becoming essential for shaping research and publishing strategies globally. 2 Learning specifics of increasingly interdisciplinary research studies and acquiring information facilitation skills aid researchers in formulating innovative ideas and predicting developments in interrelated scientific fields.

Arguably, researchers are currently offered more opportunities than in the past for generating new ideas by performing their routine laboratory activities, observing individual cases and unusual developments, and critically analyzing published scientific facts. What they need at the start of their research is to formulate a scientific hypothesis that revisits conventional theories, real-world processes, and related evidence to propose new studies and test ideas in an ethical way. 3 Such a hypothesis can be of most benefit if published in an ethical journal with wide visibility and exposure to relevant online databases and promotion platforms.

Although hypotheses are crucially important for the scientific progress, only few highly skilled researchers formulate and eventually publish their innovative ideas per se . Understandably, in an increasingly competitive research environment, most authors would prefer to prioritize their ideas by discussing and conducting tests in their own laboratories or clinical departments, and publishing research reports afterwards. However, there are instances when simple observations and research studies in a single center are not capable of explaining and testing new groundbreaking ideas. Formulating hypothesis articles first and calling for multicenter and interdisciplinary research can be a solution in such instances, potentially launching influential scientific directions, if not academic disciplines.

The aim of this article is to overview the importance and implications of infrequently published scientific hypotheses that may open new avenues of thinking and research.

Despite the seemingly established views on innovative ideas and hypotheses as essential research tools, no structured definition exists to tag the term and systematically track related articles. In 1973, the Medical Subject Heading (MeSH) of the U.S. National Library of Medicine introduced “Research Design” as a structured keyword that referred to the importance of collecting data and properly testing hypotheses, and indirectly linked the term to ethics, methods and standards, among many other subheadings.

One of the experts in the field defines “hypothesis” as a well-argued analysis of available evidence to provide a realistic (scientific) explanation of existing facts, fill gaps in public understanding of sophisticated processes, and propose a new theory or a test. 4 A hypothesis can be proven wrong partially or entirely. However, even such an erroneous hypothesis may influence progress in science by initiating professional debates that help generate more realistic ideas. The main ethical requirement for hypothesis authors is to be honest about the limitations of their suggestions. 5

EXAMPLES OF INFLUENTIAL SCIENTIFIC HYPOTHESES

Daily routine in a research laboratory may lead to groundbreaking discoveries provided the daily accounts are comprehensively analyzed and reproduced by peers. The discovery of penicillin by Sir Alexander Fleming (1928) can be viewed as a prime example of such discoveries that introduced therapies to treat staphylococcal and streptococcal infections and modulate blood coagulation. 6 , 7 Penicillin got worldwide recognition due to the inventor's seminal works published by highly prestigious and widely visible British journals, effective ‘real-world’ antibiotic therapy of pneumonia and wounds during World War II, and euphoric media coverage. 8 In 1945, Fleming, Florey and Chain got a much deserved Nobel Prize in Physiology or Medicine for the discovery that led to the mass production of the wonder drug in the U.S. and ‘real-world practice’ that tested the use of penicillin. What remained globally unnoticed is that Zinaida Yermolyeva, the outstanding Soviet microbiologist, created the Soviet penicillin, which turned out to be more effective than the Anglo-American penicillin and entered mass production in 1943; that year marked the turning of the tide of the Great Patriotic War. 9 One of the reasons of the widely unnoticed discovery of Zinaida Yermolyeva is that her works were published exclusively by local Russian (Soviet) journals.

The past decades have been marked by an unprecedented growth of multicenter and global research studies involving hundreds and thousands of human subjects. This trend is shaped by an increasing number of reports on clinical trials and large cohort studies that create a strong evidence base for practice recommendations. Mega-studies may help generate and test large-scale hypotheses aiming to solve health issues globally. Properly designed epidemiological studies, for example, may introduce clarity to the hygiene hypothesis that was originally proposed by David Strachan in 1989. 10 David Strachan studied the epidemiology of hay fever in a cohort of 17,414 British children and concluded that declining family size and improved personal hygiene had reduced the chances of cross infections in families, resulting in epidemics of atopic disease in post-industrial Britain. Over the past four decades, several related hypotheses have been proposed to expand the potential role of symbiotic microorganisms and parasites in the development of human physiological immune responses early in life and protection from allergic and autoimmune diseases later on. 11 , 12 Given the popularity and the scientific importance of the hygiene hypothesis, it was introduced as a MeSH term in 2012. 13

Hypotheses can be proposed based on an analysis of recorded historic events that resulted in mass migrations and spreading of certain genetic diseases. As a prime example, familial Mediterranean fever (FMF), the prototype periodic fever syndrome, is believed to spread from Mesopotamia to the Mediterranean region and all over Europe due to migrations and religious prosecutions millennia ago. 14 Genetic mutations spearing mild clinical forms of FMF are hypothesized to emerge and persist in the Mediterranean region as protective factors against more serious infectious diseases, particularly tuberculosis, historically common in that part of the world. 15 The speculations over the advantages of carrying the MEditerranean FeVer (MEFV) gene are further strengthened by recorded low mortality rates from tuberculosis among FMF patients of different nationalities living in Tunisia in the first half of the 20th century. 16

Diagnostic hypotheses shedding light on peculiarities of diseases throughout the history of mankind can be formulated using artefacts, particularly historic paintings. 17 Such paintings may reveal joint deformities and disfigurements due to rheumatic diseases in individual subjects. A series of paintings with similar signs of pathological conditions interpreted in a historic context may uncover mysteries of epidemics of certain diseases, which is the case with Ruben's paintings depicting signs of rheumatic hands and making some doctors to believe that rheumatoid arthritis was common in Europe in the 16th and 17th century. 18

WRITING SCIENTIFIC HYPOTHESES

There are author instructions of a few journals that specifically guide how to structure, format, and make submissions categorized as hypotheses attractive. One of the examples is presented by Med Hypotheses , the flagship journal in its field with more than four decades of publishing and influencing hypothesis authors globally. However, such guidance is not based on widely discussed, implemented, and approved reporting standards, which are becoming mandatory for all scholarly journals.

Generating new ideas and scientific hypotheses is a sophisticated task since not all researchers and authors are skilled to plan, conduct, and interpret various research studies. Some experience with formulating focused research questions and strong working hypotheses of original research studies is definitely helpful for advancing critical appraisal skills. However, aspiring authors of scientific hypotheses may need something different, which is more related to discerning scientific facts, pooling homogenous data from primary research works, and synthesizing new information in a systematic way by analyzing similar sets of articles. To some extent, this activity is reminiscent of writing narrative and systematic reviews. As in the case of reviews, scientific hypotheses need to be formulated on the basis of comprehensive search strategies to retrieve all available studies on the topics of interest and then synthesize new information selectively referring to the most relevant items. One of the main differences between scientific hypothesis and review articles relates to the volume of supportive literature sources ( Table 1 ). In fact, hypothesis is usually formulated by referring to a few scientific facts or compelling evidence derived from a handful of literature sources. 19 By contrast, reviews require analyses of a large number of published documents retrieved from several well-organized and evidence-based databases in accordance with predefined search strategies. 20 , 21 , 22

The format of hypotheses, especially the implications part, may vary widely across disciplines. Clinicians may limit their suggestions to the clinical manifestations of diseases, outcomes, and management strategies. Basic and laboratory scientists analysing genetic, molecular, and biochemical mechanisms may need to view beyond the frames of their narrow fields and predict social and population-based implications of the proposed ideas. 23

Advanced writing skills are essential for presenting an interesting theoretical article which appeals to the global readership. Merely listing opposing facts and ideas, without proper interpretation and analysis, may distract the experienced readers. The essence of a great hypothesis is a story behind the scientific facts and evidence-based data.

ETHICAL IMPLICATIONS

The authors of hypotheses substantiate their arguments by referring to and discerning rational points from published articles that might be overlooked by others. Their arguments may contradict the established theories and practices, and pose global ethical issues, particularly when more or less efficient medical technologies and public health interventions are devalued. The ethical issues may arise primarily because of the careless references to articles with low priorities, inadequate and apparently unethical methodologies, and concealed reporting of negative results. 24 , 25

Misinterpretation and misunderstanding of the published ideas and scientific hypotheses may complicate the issue further. For example, Alexander Fleming, whose innovative ideas of penicillin use to kill susceptible bacteria saved millions of lives, warned of the consequences of uncontrolled prescription of the drug. The issue of antibiotic resistance had emerged within the first ten years of penicillin use on a global scale due to the overprescription that affected the efficacy of antibiotic therapies, with undesirable consequences for millions. 26

The misunderstanding of the hygiene hypothesis that primarily aimed to shed light on the role of the microbiome in allergic and autoimmune diseases resulted in decline of public confidence in hygiene with dire societal implications, forcing some experts to abandon the original idea. 27 , 28 Although that hypothesis is unrelated to the issue of vaccinations, the public misunderstanding has resulted in decline of vaccinations at a time of upsurge of old and new infections.

A number of ethical issues are posed by the denial of the viral (human immunodeficiency viruses; HIV) hypothesis of acquired Immune deficiency Syndrome (AIDS) by Peter Duesberg, who overviewed the links between illicit recreational drugs and antiretroviral therapies with AIDS and refuted the etiological role of HIV. 29 That controversial hypothesis was rejected by several journals, but was eventually published without external peer review at Med Hypotheses in 2010. The publication itself raised concerns of the unconventional editorial policy of the journal, causing major perturbations and more scrutinized publishing policies by journals processing hypotheses.

WHERE TO PUBLISH HYPOTHESES

Although scientific authors are currently well informed and equipped with search tools to draft evidence-based hypotheses, there are still limited quality publication outlets calling for related articles. The journal editors may be hesitant to publish articles that do not adhere to any research reporting guidelines and open gates for harsh criticism of unconventional and untested ideas. Occasionally, the editors opting for open-access publishing and upgrading their ethics regulations launch a section to selectively publish scientific hypotheses attractive to the experienced readers. 30 However, the absence of approved standards for this article type, particularly no mandate for outlining potential ethical implications, may lead to publication of potentially harmful ideas in an attractive format.

A suggestion of simultaneously publishing multiple or alternative hypotheses to balance the reader views and feedback is a potential solution for the mainstream scholarly journals. 31 However, that option alone is hardly applicable to emerging journals with unconventional quality checks and peer review, accumulating papers with multiple rejections by established journals.

A large group of experts view hypotheses with improbable and controversial ideas publishable after formal editorial (in-house) checks to preserve the authors' genuine ideas and avoid conservative amendments imposed by external peer reviewers. 32 That approach may be acceptable for established publishers with large teams of experienced editors. However, the same approach can lead to dire consequences if employed by nonselective start-up, open-access journals processing all types of articles and primarily accepting those with charged publication fees. 33 In fact, pseudoscientific ideas arguing Newton's and Einstein's seminal works or those denying climate change that are hardly testable have already found their niche in substandard electronic journals with soft or nonexistent peer review. 34

CITATIONS AND SOCIAL MEDIA ATTENTION

The available preliminary evidence points to the attractiveness of hypothesis articles for readers, particularly those from research-intensive countries who actively download related documents. 35 However, citations of such articles are disproportionately low. Only a small proportion of top-downloaded hypotheses (13%) in the highly prestigious Med Hypotheses receive on average 5 citations per article within a two-year window. 36

With the exception of a few historic papers, the vast majority of hypotheses attract relatively small number of citations in a long term. 36 Plausible explanations are that these articles often contain a single or only a few citable points and that suggested research studies to test hypotheses are rarely conducted and reported, limiting chances of citing and crediting authors of genuine research ideas.

A snapshot analysis of citation activity of hypothesis articles may reveal interest of the global scientific community towards their implications across various disciplines and countries. As a prime example, Strachan's hygiene hypothesis, published in 1989, 10 is still attracting numerous citations on Scopus, the largest bibliographic database. As of August 28, 2019, the number of the linked citations in the database is 3,201. Of the citing articles, 160 are cited at least 160 times ( h -index of this research topic = 160). The first three citations are recorded in 1992 and followed by a rapid annual increase in citation activity and a peak of 212 in 2015 ( Fig. 1 ). The top 5 sources of the citations are Clin Exp Allergy (n = 136), J Allergy Clin Immunol (n = 119), Allergy (n = 81), Pediatr Allergy Immunol (n = 69), and PLOS One (n = 44). The top 5 citing authors are leading experts in pediatrics and allergology Erika von Mutius (Munich, Germany, number of publications with the index citation = 30), Erika Isolauri (Turku, Finland, n = 27), Patrick G Holt (Subiaco, Australia, n = 25), David P. Strachan (London, UK, n = 23), and Bengt Björksten (Stockholm, Sweden, n = 22). The U.S. is the leading country in terms of citation activity with 809 related documents, followed by the UK (n = 494), Germany (n = 314), Australia (n = 211), and the Netherlands (n = 177). The largest proportion of citing documents are articles (n = 1,726, 54%), followed by reviews (n = 950, 29.7%), and book chapters (n = 213, 6.7%). The main subject areas of the citing items are medicine (n = 2,581, 51.7%), immunology and microbiology (n = 1,179, 23.6%), and biochemistry, genetics and molecular biology (n = 415, 8.3%).

Interestingly, a recent analysis of 111 publications related to Strachan's hygiene hypothesis, stating that the lack of exposure to infections in early life increases the risk of rhinitis, revealed a selection bias of 5,551 citations on Web of Science. 37 The articles supportive of the hypothesis were cited more than nonsupportive ones (odds ratio adjusted for study design, 2.2; 95% confidence interval, 1.6–3.1). A similar conclusion pointing to a citation bias distorting bibliometrics of hypotheses was reached by an earlier analysis of a citation network linked to the idea that β-amyloid, which is involved in the pathogenesis of Alzheimer disease, is produced by skeletal muscle of patients with inclusion body myositis. 38 The results of both studies are in line with the notion that ‘positive’ citations are more frequent in the field of biomedicine than ‘negative’ ones, and that citations to articles with proven hypotheses are too common. 39

Social media channels are playing an increasingly active role in the generation and evaluation of scientific hypotheses. In fact, publicly discussing research questions on platforms of news outlets, such as Reddit, may shape hypotheses on health-related issues of global importance, such as obesity. 40 Analyzing Twitter comments, researchers may reveal both potentially valuable ideas and unfounded claims that surround groundbreaking research ideas. 41 Social media activities, however, are unevenly distributed across different research topics, journals and countries, and these are not always objective professional reflections of the breakthroughs in science. 2 , 42

Scientific hypotheses are essential for progress in science and advances in healthcare. Innovative ideas should be based on a critical overview of related scientific facts and evidence-based data, often overlooked by others. To generate realistic hypothetical theories, the authors should comprehensively analyze the literature and suggest relevant and ethically sound design for future studies. They should also consider their hypotheses in the context of research and publication ethics norms acceptable for their target journals. The journal editors aiming to diversify their portfolio by maintaining and introducing hypotheses section are in a position to upgrade guidelines for related articles by pointing to general and specific analyses of the subject, preferred study designs to test hypotheses, and ethical implications. The latter is closely related to specifics of hypotheses. For example, editorial recommendations to outline benefits and risks of a new laboratory test or therapy may result in a more balanced article and minimize associated risks afterwards.

Not all scientific hypotheses have immediate positive effects. Some, if not most, are never tested in properly designed research studies and never cited in credible and indexed publication outlets. Hypotheses in specialized scientific fields, particularly those hardly understandable for nonexperts, lose their attractiveness for increasingly interdisciplinary audience. The authors' honest analysis of the benefits and limitations of their hypotheses and concerted efforts of all stakeholders in science communication to initiate public discussion on widely visible platforms and social media may reveal rational points and caveats of the new ideas.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Gasparyan AY, Yessirkepov M, Kitas GD.
• Methodology: Gasparyan AY, Mukanova U, Ayvazyan L.
• Writing - original draft: Gasparyan AY, Ayvazyan L, Yessirkepov M.
• Writing - review & editing: Gasparyan AY, Yessirkepov M, Mukanova U, Kitas GD.