The Kitchen Pantry Scientist

Simple recipes for real science, diffusion and osmosis experiments.

experiments to show osmosis and diffusion

Think about the way pollutants move from one place to another through air, water and even soil. Or consider how bacteria are able to take up the substances they need to thrive. Your body has to transfer oxygen, carbon dioxide and water by processes involving diffusion as well.

Lots of things can affect how fast molecules diffuse, including temperature.  When molecules are heated up, they vibrate faster and move around faster, which helps them achieve equilibrium more quickly than they would if it were cold.

Diffusion takes place in gases (like air), liquids (like food coloring moving through water,) and even solids (semiconductors for computers are made by diffusing elements into one another.)

experiments to show osmosis and diffusion

Every so often, measure the circle of food coloring as it diffuses into the jello around it.  How many cm per hour is it diffusing?  If you put one plate in the refrigerator and an identical one at room temperature, do they diffuse at the same rate?  Why do you think you see more than one color for certain shades of food coloring? What else could you try?

Here’s a post on how to use this experiment to make sticky window decorations:   https://kitchenpantryscientist.com/?p=4489

We made plates and did the same experiment using 2 cups of red cabbage juice , 2 cups of water and 4 packs of gelatin to see how fast a few drops of vinegar or baking soda solution would diffuse (a pigment in red cabbage turns pink when exposed to acid, and blue/green when exposed to a base!)

experiments to show osmosis and diffusion

It’s also fun to experiment with the diffusion of substances across a membrane, like a paper towel.  This is called osmosis. Membranes like the ones around your cells are selectively permeable and let water and oxygen in and out, but keep other, larger molecules from freely entering and exiting your cells.

For this experiment, you’ll need a jar (or two), paper towels, rubber bands and food coloring.  Fill a jar with water and secure a paper towel in the jar’s mouth (with a rubber band) so that it hangs down into the water, making a water-filled chamber that you can add food coloring to.  Put a few drops of food coloring into the chamber and see what happens.

experiments to show osmosis and diffusion

top “chambers” for food coloring

experiments to show osmosis and diffusion

Are the food coloring molecules small enough to pass through the paper towel “membrane?”  What happens if you put something bigger, like popcorn kernels in the chamber? Can they pass through the small pores in the paper towel?

Do the same experiment in side-by-side jars, but fill one with ice water and the other with hot  water.  Does this affect the rate of osmosis or how fast the food coloring molecules diffuse throughout the water?

Think about helium balloons.  If you take identical balloons and fill one with helium and the other with air, the helium balloon will shrink much faster as the smaller helium atoms diffuse out more quickly than the larger oxygen molecules.

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6 EASY ways to model OSMOSIS

experiments to show osmosis and diffusion

Teaching osmosis?

Osmosis is one of my favorite topics to teach during the cells unit because it’s so easily visualized. There are quite a few ways to easily see osmosis in action! Here’s a round-up of six labs and the pros and cons of each:

1. EGG OSMOSIS

The “naked egg” lab is very popular in biology. Students really get a kick out of seeing the eggshell dissolve! In this lab, students dissolve the shell of an egg by soaking it in vinegar (this takes about 3 days, so its best to set up on a Friday). Once the shell is gone, students carefully transfer their naked eggs to hypotonic distilled water, and a hypertonic solution like corn syrup or molasses. Students compare the mass of the egg before and after soaking and figure out which way the water moved while attempting to reach equilibrium.

experiments to show osmosis and diffusion

PROS: Students love this lab! Also, eggs are round like red blood cells, so they can visualize what happens to cells in different hypertonic or hypotonic solutions.

CONS: It can get expensive to purchase enough eggs for all of your classes. Also, expect some to break along the way, so soak some extras just in case. Overall, it feels a little wasteful of perfectly good eggs. Maybe do this one as a demonstration, and then choose one of the options below for students to do in groups.

2. PURPLE ONION SKIN

Of all the osmosis labs, this one might be my favorite because I’m partial to getting out the microscopes. In this lab, students get a small piece of onion skin and make a wet mount slide using fresh water. They will see nice rectangular purple onion cells. Next, they will swap out the fresh water for salt water, and watch the cytoplasm in each cell shrivel up. If your students already know how to use microscopes, this one is a hit! Here is a full blog post with more details and pictures.

Note: I used to use elodea for this lab, but it became difficult to find at pet stores since it is invasive in many states. Purple onion is easy and cheaper! You can also find a lab write-up here .

experiments to show osmosis and diffusion

PROS: Inexpensive, and fool proof. It is impossible to mess up! Unlike other options, this lab uses real plant cells. Also, it is the only version that doesn’t require soaking things overnight so you can get it done in one class period.

CONS: Your classroom will smell like onion for a day.

3. DIALYSIS TUBING

This version is a little fancier, and is a great option if you have honors or AP students. In this lab, students will get dialysis tubing and fill them with varying concentrations of sugar water solution. They will measure the initial mass of the tubes, and then soak the dialysis tubing overnight in distilled water. The following day they will measure the new mass, and see how water moved across the dialysis tubing membrane. You can find a free version of this lab from Amy Brown on TpT.

PROS: Students collect quantitative data, and it feels more “scientific” than other options.

CONS: Dialysis tubing is expensive, and if students don’t tie the string tight enough they can leak.

experiments to show osmosis and diffusion

4. GUMMY BEARS

I am the first to admit gummy bears are not my favorite option, but I know many teachers who love doing it this way. In this version, students soak gummy bears in tap water, distilled water, and salt water overnight. They measure the change in the size of the gummy bear using rulers.

PROS: A large bag of gummy bears is only a few dollars, and students always love working with candy.

experiments to show osmosis and diffusion

CONS: I don’t love this lab because depending on the brand you buy, the gummy bears can begin to dissolve and fall apart. Also, since they are an irregular shape it is difficult to calculate the change in volume.

5. WATER BEADS

An alternative to gummy bears is water beads, or Orbeez. You set it up the same way by soaking them in fresh water and salt water. If your kid already has some at home, use them instead of gummy bears. I like them better for a few reasons:

PROS: Water beads won’t fall apart like gummy bears, even after soaking multiple days. Since they are round, you can have students measure the diameter, and calculate the volume of the sphere. Also, you can dry them out afterward, place them in a Tupperware, and re-use them the following year.

experiments to show osmosis and diffusion

CONS: Orbeez are smaller than gummy bears, so if your students struggle taking small, accurate measurements that might be a point of struggle. (Note: Beware of cheaper off-brands you can find on Amazon, because they WILL fall part unlike Orbeez).

6. BABY CARROTS

Last but not least are baby carrots! In this lab, each lab group gets 2 baby carrots. Just like other labs, they measure the mass before and after soaking them in fresh and salt water. Students will also notice that the baby carrot soaked in salt water becomes flimsy and bendable overnight. If you have already covered organelles, this can lead to a discussion about vacuoles and how plant cells become limp and flimsy when they lose water. You can find a lab write-up on my website !

experiments to show osmosis and diffusion

PROS: Inexpensive materials, and uses real plant cells.

CONS: You won’t see the size of the carrot change, only the flexible vs stiff texture.

Alright, which is your favorite? Choose one (or two) and have a blast!

experiments to show osmosis and diffusion

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Osmosis Experiment: Dissolving Egg Shells With Vinegar

How does osmosis keep you healthy.

Right now, as you read this, there are millions of things happening throughout your body. The food you ate just a bit ago is making its way through a watery slurry inside your stomach and small intestines. Your kidneys are working hard to excrete waste and extra water. The lacrimal glands near your eyes are secreting tears, which allow your eyelids to close without damaging your eyeballs. What’s one thing that all of these processes have in common? They all rely on osmosis: the diffusion of water from one place to another.

Osmosis factors heavily in each of these processes and is an important force for keeping every single cell in your body healthy. Osmosis is hard to see without a microscope. But if we create our very own model of a cell, using a shell-less chicken egg, we can see what happens when we manipulate the osmotic balance in the “cell”!

dissolving-egg-shell-experiment-4

  • 3 glasses (large enough to fit the egg plus liquid)
  • 3 butter knives
  • White vinegar (about 3 cups)
  • Distilled water (about 2 cups)
  • Light corn syrup (about 1 ¼ cups)
  • Slotted spoon
  • Measuring cup (1 cup)
  • Measuring spoons (1 tablespoon and ½ tablespoon)
  • Sticky notes and marker
  • Scale (optional)

Note : It’s okay to touch the eggs, but remember to wash your hands afterwards to avoid any nasty surprises!

1. Place one egg in each glass. Pour in enough vinegar to cover each egg. Bubbles will start to form around the egg, and it’ll float up. To keep it submerged, put a butter knife in the glass to hold it down.

2. Put the three glasses in the refrigerator and allow to sit for 24 hours.

3. Gently holding the egg in the glass, pour out the old vinegar. Replace with fresh vinegar, and let sit in the refrigerator for another 24 hours. Repeat this process until the shells are fully dissolved and only the membrane remains. This should take about 2-3 days.

4. Gently remove the eggs using the slotted spoon and rinse with tap water in the sink. Rinse out the empty glasses as well.

5. Gently put the shell-less eggs aside for a moment on a plate.

6. Prepare three different sugar-water solutions as follows, labeling with sticky notes:

Glass 1: Label “hypertonic”. Pour in one cup of corn syrup.

Glass 2: Label “isotonic”. Add 1 ½ tablespoons corn syrup to the one cup measuring cup, and fill the remainder with distilled water. Pour into glass (make sure you get all the corn syrup out!) and stir to dissolve.

Glass 3: Label “hypotonic”. Pour in one cup of distilled water. Gently put one shell-less egg in each of the glasses, and let sit in the refrigerator for another 24 hours.

osmosis-graph

7. Remove the glasses from the refrigerator, and gently put the eggs on a plate. If you weighed the eggs before putting them in each solution, weigh them again. What happened to each of the eggs?

egg-experiment-3

How does osmosis work?

Osmosis is the scientific term that describes how water flows to different places depending on certain conditions. In this case, water moves around to different areas based on a concentration gradient , i.e. solutions which have different concentrations of dissolved particles ( solutes ) in them. Water always flows to the area with the most dissolved solutes, so that in the end both solutions have an equal concentration of solutes. Think about if you added a drop of food dye to a cup of water – even if you didn’t stir it, it would eventually dissolve on its own into the water.

In biological systems, the different solutions are usually separated by a semipermeable membrane , like cell membranes or kidney tubules . These act sort of like a net that keeps solutes trapped, but they still allow water to pass through freely. In this way, cells can keep all of their “guts” contained but still exchange water.

Now, think about the inside of an egg. There’s a lot of water inside of the egg, but a lot of other things (i.e. solutes) too, like protein and fat. When you placed the egg in the three solutions, how do you think the concentration of solutes differed between the inside of the egg and outside of the egg? The egg membrane acts as a semipermeable membrane and keeps all of the dissolved solutes separated but allows the water to pass through.

How did osmosis make the eggs change size (or not)?

If the steps above work out properly, the results should be as follows.

In the case of the hypertonic solution, there were more solutes in the corn syrup than there were in the egg. So, water flowed out of the egg and into the corn syrup, and as a result the egg shriveled up.

In the case of the isotonic solution, there was roughly an equal amount of solutes in the corn syrup/water solution than there was in the egg, so there was no net movement in or out of the egg. It stayed the same size.

In the case of the hypotonic solution, there were more solutes in the egg than in the pure water. So, water flowed into the egg, and as a result, it grew in size.

experiments to show osmosis and diffusion

Osmosis and You

Every cell in your body needs the right amount of water inside of it to keep its shape, produce energy, get rid of wastes, and other functions that keep you healthy.

This is why medicines that are injected into patients need to be carefully designed so that the solution has the same concentration of solutes as their cells (i.e. isotonic). If you were sick and became dehydrated, for example, you would get a 0.90% saline IV drip. If it were too far off from this mark it wouldn’t be isotonic anymore, and your blood cells might shrivel up or even explode , depending on the concentration of dissolved solutes in the water.

Osmosis works just the same way in your cells as it does in our egg “cell” model. Thankfully, though, the semipermeable membrane of the egg is much stronger, so you don’t have to worry about the egg exploding as well!

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Lindsay graduated with a master’s degree in wildlife biology and conservation from the University of Alaska Fairbanks. She also spent her time in Alaska racing sled dogs, and studying caribou and how well they are able to digest nutrients from their foods. Now, she enjoys sampling fine craft beers in Fort Collins, Colorado, knitting, and helping to inspire people to learn more about wildlife, nature, and science in general.

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Observing Diffusion Using Iodine and Plastic Bags

experiments to show osmosis and diffusion

Most chapters follow the cell structure topic with one on the cell membrane and diffusion and osmosis. These concepts can be very difficult for students to understand.

In order to give them a view of how diffusion works with a semipermeable membrane, I like to do a lab that uses a plastic bag to model the cell (membrane).

It is a simple lab where students do very little except watch the process and record data and information.   To set it up, you will need plastic bags, iodine, water, and corn starch.  All except iodine are readily available at the supermarket.

Student handout includes instructions, pre-lab questions, and an analysis section.

Setting Up the Lab

First, add a spoonful of cornstarch and about 100 ml of water in a cheap plastic bag. (You don’t need to be exact!)  Tie the top of the bag like a balloon. I like to do at least one of these while the students watch. Then I give them prepared bags because it takes too long (and is messy) for students to do this step.   

experiments to show osmosis and diffusion

Tell the students that the bag represents a cell, with the cytoplasm being the cornstarch mixture and the plastic is the cell membrane.   Explain that solid objects are not really solid at a molecular level and that the bag is more like a tiny little screen door.  If molecules are small enough, they can pass right through the bag.

With the baggie in place, you will need to prepare enough beakers for your entire class.  Fill them about half full and add several drops of iodine , You want the water to be very orange.  The more concentrated the mixture, the faster the reaction.

experiments to show osmosis and diffusion

Observations and Analysis

Explain that iodine is an “INDICATOR” in that it will change color whenever it encounters starch.  You can demonstrate this with a beaker of starch solution and a drop of  iodine. Usually students are fascinated by the quick and dramatic color change!

Finally, students will carefully place the baggie into the iodine mixture.  Their worksheet will ask them to make some predictions about what will happen and to define diffusion and osmosis.  The process should take about 15 minutes and students should notice a change in the color of the corn starch in the bag.

experiments to show osmosis and diffusion

After 15 Minutes….

experiments to show osmosis and diffusion

Students will then be asked on the worksheet to explain what happened.  A common misconception is that the iodine “ate” through the bag.  Remind students that the bag is like a screen door and iodine is a very small molecule.

Despite the fact that this lab is not very interactive, students do seem to understand the cell model and semi-permeable membranes after completing it.  Many will ask to see what will happen if you put the starch in the beaker and the iodine in the bag. You can set this up for them and they can view the results the next day.

Shannan Muskopf

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Simple Candy Osmosis Experiment

Demonstrate Osmosis Using Gummy Bears

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Osmosis is the diffusion of water across a semipermeable membrane. The water moves from an area of higher to lower solvent concentration (an area of lower to higher solute concentration). It's an important passive transport process in living organisms, with applications to chemistry and other sciences. You don't need fancy lab equipment to observe osmosis. You can experiment with the phenomenon using gummy bears and water. Here's what you do:

Osmosis Experiment Materials

Basically, all you need for this chemistry project are colored candies and water:

  • Gummy bear candies (or other gummy candy)
  • Plate or shallow bowl

The gelatin of the gummy candies acts as a semipermeable membrane . Water can enter the candy, but it's much harder for sugar and coloring to leave exit it.

What You Do

It's easy! Simply place one or more of the candies in the dish and pour in some water. Over time, water will enter the candies, swelling them. Compare the size and "squishiness" of these candies with how they looked before. Notice the colors of the gummy bears starts to appear lighter. This is because the pigment molecules (solute molecules) are being diluted by the water (solvent molecules) as the process progresses.

What do you think would happen if you used a different solvent, such as milk or honey, that already contains some solute molecules? Make a prediction, then try it and see.

How do you think osmosis in a gelatin dessert compares with osmosis in candy? Again, make a prediction and then test it!

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Osmosis and Diffusion Lab

This lab allows you to learn about two forms of passive transport: diffusion and osmosis. You will compare and contrast similarities and differences in the processes of diffusion and osmosis. Use a colorimetric test to demonstrate the movement of a solute across a semi-permeable membrane. Set up an environment likely to facilitate osmosis and gather data to determine whether or not osmosis may have occurred. Kit contains enough materials for 15 groups.

Lesson Files

  • Osmosis and Diffusion Teachers Manual (.pdf)
  • Osmosis and Diffusion Student Guide (.pdf)

Materials Needed for this Lesson Plan

  • Osmosis and Diffusion Lab Kit
  • Electronic balance
  • Small funnels or pipettes
  • Safety goggles
  • lab apron (optional)
  • Distilled or deionized water
  • Paper towels

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Using potatoes for osmosis and diffusion labs.

experiments to show osmosis and diffusion

  • Teach or illustrate multiple concepts
  • Use simple consumable materials and readily available lab items
  • Involve weighing, measuring and calculating
  • Require the students to make a HAND-MADE graph
  • Provide a perfect visual of the concepts I teach in the classroom
  • Reinforce the use of lab equipment and proper laboratory skills
  • Require the students to use critical thinking and problem solving skills
  • Require students to closely examine the date in order to draw a conclusion

experiments to show osmosis and diffusion

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IMAGES

  1. Diffusion and Osmosis experiments « The Kitchen Pantry Scientist

    experiments to show osmosis and diffusion

  2. Diffusion and Osmosis Activities and Experiments

    experiments to show osmosis and diffusion

  3. Very Simple Diffusion and Osmosis Experiment

    experiments to show osmosis and diffusion

  4. Simple water experiments to explain osmosis and diffusion....

    experiments to show osmosis and diffusion

  5. Osmosis and Diffusion experiments in Maple Class

    experiments to show osmosis and diffusion

  6. Diffusion and Osmosis experiments « The Kitchen Pantry Scientist

    experiments to show osmosis and diffusion

VIDEO

  1. BongMistry: The Ultimate Diffusion Experiment

  2. The Potato Osmosis Experiment! 🥔#Science #STEM #ScienceExperiments #Osmosis #Shorts

  3. Osmosis

  4. Diffusion of KMno4 in hot and cold water #diffusion #science

  5. OSMOSIS VS DIFFUSION #science 9TH CLASS BIOLOGY || NEET BIOLOGY @UjmaBiologyofficial

  6. What is Osmosis? Science for Kids! #shorts

COMMENTS

  1. Very Simple Diffusion and Osmosis Experiment - Amy Brown Science

    Very Simple Diffusion and Osmosis Experiment. The concept of cellular transport (diffusion, osmosis, hypotonic, hypertonic, active transport, passive transport) is fundamental to a biology class. There are so many great ideas for labs that teach and explore these concepts.

  2. Diffusion and Osmosis experiments - Kitchen Pantry Scientist

    It’s also fun to experiment with the diffusion of substances across a membrane, like a paper towel. This is called osmosis. Membranes like the ones around your cells are selectively permeable and let water and oxygen in and out, but keep other, larger molecules from freely entering and exiting your cells.

  3. 6 easy ways to model osmosis! - Science Lessons That Rock

    There are quite a few ways to easily see osmosis in action! Here’s a round-up of six labs and the pros and cons of each: 1. EGG OSMOSIS. The “naked egg” lab is very popular in biology. Students really get a kick out of seeing the eggshell dissolve! In this lab, students dissolve the shell of an egg by soaking it in vinegar (this takes ...

  4. Osmosis Experiment: Dissolve an Egg Shell [The Lab]

    Osmosis Experiment: Dissolving Egg Shells With Vinegar. How does osmosis keep you healthy? Right now, as you read this, there are millions of things happening throughout your body. The food you ate just a bit ago is making its way through a watery slurry inside your stomach and small intestines.

  5. Diffusion Lab - The Biology Corner

    Observing Diffusion Using Iodine and Plastic Bags. Most chapters follow the cell structure topic with one on the cell membrane and diffusion and osmosis. These concepts can be very difficult for students to understand.

  6. Simple Candy Osmosis Experiment - ThoughtCo

    Osmosis is the diffusion of water across a semipermeable membrane. Use gummy bears to demonstrate osmosis with this simple science experiment.

  7. Module 4: Diffusion and Osmosis - Biology LibreTexts

    To understand this process you need to understand the makeup of the cell membrane and an important phenomenon known as diffusion. Diffusion is the movement of a substance from an area of high concentration to an area of low concentration due to random molecular motion.

  8. 1.6: Diffusion and Osmosis - Biology ... - Biology LibreTexts

    Exercise 1: Diffusion Through a Gel. One factor that can affect the rate of diffusion is the size of the molecule. Larger molecules tend to move more slowly than smaller molecules. In this experiment, students will compare the diffusion rates of two dyes traveling through agar.

  9. Osmosis and Diffusion Lab - STEM Library Lab

    This lab allows you to learn about two forms of passive transport: diffusion and osmosis. You will compare and contrast similarities and differences in the processes of diffusion and osmosis. Use a colorimetric test to demonstrate the movement of a solute across a semi-permeable membrane.

  10. Using Potatoes for Osmosis and Diffusion Labs - Amy Brown Science

    Using Potatoes for Osmosis and Diffusion Labs. • Easy materials list! • Easy set up and clean up! •Involves calculations and graphing! Win! I love labs that: Teach or illustrate multiple concepts. Use simple consumable materials and readily available lab items. Involve weighing, measuring and calculating.