A Beginner's Guide to Cell Culture: Practical Advice for Preventing Needless Problems
1. Introduction. Cell culture experiments are widely used in biomedical research, regenerative medicine, and biotechnological production. Due to restrictions on the use of laboratory animals by animal protection laws and the strict implementation of the 3Rs (Replacement, Reduction, and Refinement) formulated by William Russell and Rex Burch to improve the welfare of animals, it can be expected ...
Cell Culture
Cell culture is a very versatile tool in the investigation of basic scientific and translation research questions. The advantage of using cell lines in scientific research is their homogeneity and associated reproducibility in data generated. This chapter introduces the principles behind the setup of a cell culture lab and the guidelines that ...
Cell Culture, Technology: Enhancing the Culture of Diagnosing Human
Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in vitro).Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic ...
Cell culture
Cell culture is a method for growing or maintaining cells in vitro under controlled conditions. Primary cell cultures refer to dispersed cells that are cultured directly from tissues and have ...
Recent Advances in Three‐Dimensional Stem Cell Culture Systems and
1. Introduction. Cell culture is one of the most important and fundamental techniques in the fields of biology and medicine. It involves extracting cells from biological tissues, simulating the survival environment in vivo to ensure their growth and reproduction, and maintaining their main structures and functions under sterile conditions with suitable temperature, pH, and adequate nutrient ...
A Beginner's Guide to Cell Culture: Practical Advice for ...
Abstract. The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently ...
A Beginner's Guide to Cell Culture: Practical Advice for Preventing
This review article provides a beginner's guide to cell culture, covering common problems, contaminants, misidentification, and ethical issues. It does not address the use of animals in research or cell culture, which is the query of the web page.
Cell culture
This paper reports culture conditions for the expansion of near-homogeneous populations of mouse Lgr5 + intestinal stem cells. These methods will enable the study of intestinal biology and ...
Cells
Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. ... Advanced human cell culture ...
Importance of Primary Cell Culture in Biomedical Research
Special Issue Information. Dear Colleagues, Primary cell culture plays an important role in biomedical research. Changes in culturing conditions can regulate the biological behavior and performance of the cells, which can induce the cells into a more suitable state for use. Common methods include adding many kinds of regulating factors to the ...
Cell culture: in vitro model system and a promising path to in vivo
Cell culture is defined as a method to grow cells in a suitable physiological condition, including media, temperature, and potential of hydrogen (pH). Since the early last century, cell culture has been widely used for virus study, vaccine, gene function, and pharmaceutical productions. In basic research, cells are routinely used in studying ...
Introduction to cell culture
Abstract. The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture.
Toward Best Practices for Controlling Mammalian Cell Culture
Introduction. Mammalian cell cultures have been a foundational resource in almost every biomedical research program since the 1990s (Petricciani, 1995; Hu and Aunins, 1997; Merten, 2006).The use of mammalian cell cultures as preclinical models ranges from the characterization of in vivo physiological mechanisms and manipulation of disease-related pathways to the maintenance of stem cells for ...
(PDF) A Brief Concept of Cell Culture: Challenges, Prospects and
General concept of cell culture is the prop agation o f cells or fibrob last or livin g. tissues in a defined media that conduciv e their growth. Shortly, a growth of cell. artificially known as ...
Recent Advances on Cell Culture Platforms for In Vitro Drug Screening
Furthermore, paper is advantageous concerning cell-based assays due to its reticulated structure, which offers a naturally 3D environment closer to the native cellular environment. ... This review highlights the fundamental role that cell culture methods play in life research and development, as well as recent discoveries and technological ...
Research review paper Challenges in developing cell culture media using
1. Experimental design: Clarify the purpose of cell culture and determine the medium components and the scales of their changes for cell culture experiments. 2. Data acquisition: Perform cell culture experiments to obtain the dataset that links cell culture goodness to the medium composition. 3.
Paper-based cell culture platform and its emerging biomedical
The unique features of paper-based system in supporting 3D cell culture enables it to be used for a range of studies, such as the development of normal or disease models in vitro by manipulating the physical or chemical properties of the paper to control nutrient and oxygen diffusion (Fig. 1) [9], [10].Such 3D cell culture models can also be used to study cell-drug interactions in a high ...
Cell Cultures for Virology: Usability, Advantages, and Prospects
There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity ...
Animal‐cell culture media: History, characteristics, and current issues
1. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of vaccines and ...
Advances in Cell Culture and Tissue Engineering
Topical Collection Information. Dear Colleagues, Cell culture systems are one of the most important tools in cellular and molecular biology, tissue engineering, drug discovery, and beyond. They are essential to address basic research questions, and to pave the way for therapeutic approaches. One crucial requirement of cell culture systems is ...
Maximizing the relevance and reproducibility of A549 cell culture using
A549 cells, a human lung alveolar-like cell line, were transitioned from FBS-supplemented media to FBS-free media. The study assessed the genotype, morphology, and functionality of cells grown in X-VIVO™ 10 and CnT-Prime Airway media under submerged and air-liquid-interface conditions.
3D printing of hydrogel for cell engineering
Lab-on-chips supported by hydrogel matrices are excellent solutions for cell culture; thus, this literature review presents examples of scientific research in this area. Several works are presenting the properties of biocompatible hydrogels that mimic the cellular environment published recently. Hydrogels can also be treated as cell transporters or as a structural component of microfluidic ...
Animal tissue culture principles and applications
Monitoring cell growth. The animal cell culture can be grown for a wide variety of cell-based assays to investigate morphology, protein expression, cell growth, differentiation, apoptosis, and toxicity in different environments. Product yields can be increased if monitoring of cell growth is managed properly.
Biomedicines
Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. ... (3D) cell culture to assess the ...
In vitro plant tissue culture: means for production of biological
Plant cell and tissue culture uses nutritive culture media and controlled aseptic conditions for the growth of plant cells, tissues and organs. Since its first establishment by Haberlandt in the early twentieth century, this type of culture has evolved into an essential tool for plant research at both the basic and applied levels (Haberlandt ...
COMMENTS
1. Introduction. Cell culture experiments are widely used in biomedical research, regenerative medicine, and biotechnological production. Due to restrictions on the use of laboratory animals by animal protection laws and the strict implementation of the 3Rs (Replacement, Reduction, and Refinement) formulated by William Russell and Rex Burch to improve the welfare of animals, it can be expected ...
Cell culture is a very versatile tool in the investigation of basic scientific and translation research questions. The advantage of using cell lines in scientific research is their homogeneity and associated reproducibility in data generated. This chapter introduces the principles behind the setup of a cell culture lab and the guidelines that ...
Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in vitro).Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic ...
Cell culture is a method for growing or maintaining cells in vitro under controlled conditions. Primary cell cultures refer to dispersed cells that are cultured directly from tissues and have ...
1. Introduction. Cell culture is one of the most important and fundamental techniques in the fields of biology and medicine. It involves extracting cells from biological tissues, simulating the survival environment in vivo to ensure their growth and reproduction, and maintaining their main structures and functions under sterile conditions with suitable temperature, pH, and adequate nutrient ...
Abstract. The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently ...
This review article provides a beginner's guide to cell culture, covering common problems, contaminants, misidentification, and ethical issues. It does not address the use of animals in research or cell culture, which is the query of the web page.
This paper reports culture conditions for the expansion of near-homogeneous populations of mouse Lgr5 + intestinal stem cells. These methods will enable the study of intestinal biology and ...
Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. ... Advanced human cell culture ...
Special Issue Information. Dear Colleagues, Primary cell culture plays an important role in biomedical research. Changes in culturing conditions can regulate the biological behavior and performance of the cells, which can induce the cells into a more suitable state for use. Common methods include adding many kinds of regulating factors to the ...
Cell culture is defined as a method to grow cells in a suitable physiological condition, including media, temperature, and potential of hydrogen (pH). Since the early last century, cell culture has been widely used for virus study, vaccine, gene function, and pharmaceutical productions. In basic research, cells are routinely used in studying ...
Abstract. The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture.
Introduction. Mammalian cell cultures have been a foundational resource in almost every biomedical research program since the 1990s (Petricciani, 1995; Hu and Aunins, 1997; Merten, 2006).The use of mammalian cell cultures as preclinical models ranges from the characterization of in vivo physiological mechanisms and manipulation of disease-related pathways to the maintenance of stem cells for ...
General concept of cell culture is the prop agation o f cells or fibrob last or livin g. tissues in a defined media that conduciv e their growth. Shortly, a growth of cell. artificially known as ...
Furthermore, paper is advantageous concerning cell-based assays due to its reticulated structure, which offers a naturally 3D environment closer to the native cellular environment. ... This review highlights the fundamental role that cell culture methods play in life research and development, as well as recent discoveries and technological ...
1. Experimental design: Clarify the purpose of cell culture and determine the medium components and the scales of their changes for cell culture experiments. 2. Data acquisition: Perform cell culture experiments to obtain the dataset that links cell culture goodness to the medium composition. 3.
The unique features of paper-based system in supporting 3D cell culture enables it to be used for a range of studies, such as the development of normal or disease models in vitro by manipulating the physical or chemical properties of the paper to control nutrient and oxygen diffusion (Fig. 1) [9], [10].Such 3D cell culture models can also be used to study cell-drug interactions in a high ...
There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity ...
1. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of vaccines and ...
Topical Collection Information. Dear Colleagues, Cell culture systems are one of the most important tools in cellular and molecular biology, tissue engineering, drug discovery, and beyond. They are essential to address basic research questions, and to pave the way for therapeutic approaches. One crucial requirement of cell culture systems is ...
A549 cells, a human lung alveolar-like cell line, were transitioned from FBS-supplemented media to FBS-free media. The study assessed the genotype, morphology, and functionality of cells grown in X-VIVO™ 10 and CnT-Prime Airway media under submerged and air-liquid-interface conditions.
Lab-on-chips supported by hydrogel matrices are excellent solutions for cell culture; thus, this literature review presents examples of scientific research in this area. Several works are presenting the properties of biocompatible hydrogels that mimic the cellular environment published recently. Hydrogels can also be treated as cell transporters or as a structural component of microfluidic ...
Monitoring cell growth. The animal cell culture can be grown for a wide variety of cell-based assays to investigate morphology, protein expression, cell growth, differentiation, apoptosis, and toxicity in different environments. Product yields can be increased if monitoring of cell growth is managed properly.
Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. ... (3D) cell culture to assess the ...
Plant cell and tissue culture uses nutritive culture media and controlled aseptic conditions for the growth of plant cells, tissues and organs. Since its first establishment by Haberlandt in the early twentieth century, this type of culture has evolved into an essential tool for plant research at both the basic and applied levels (Haberlandt ...